human preadipocytes Search Results


htert human pre adipocytes  (ATCC)
92
ATCC htert human pre adipocytes
( A – C ) <t>hTERT</t> pre-adipocytes and differentiated adipocytes infected with SARS-CoV-2 (WA1/2020, MOI = 1). ( A , B ) Relative expression of viral ( A ) genomic RNA (nucleocapsid, *** p = 0.0002, ** p = 0.0018) and ( B ) sub-genomic RNA (ORF1ab, *** p = 0.0003, * p = 0.0263), normalized to β-actin. ( C ) Viral loads measured from supernatant using plaque assay. (**** p < 0.0001, ** p = 0.0024). Data are pooled from two independent experiments ( n = 8, biological replicates). Statistical analysis was performed using two-way ANOVA. ( D ) IL-6 levels in the supernatant of differentiated adipocytes with or without viral infection (MOI = 1), measured by ELISA. Data are pooled from three independent experiments ( n = 14, biological replicates, **** p < 0.0001). Statistical analysis was performed using one-way ANOVA. ( E – G ) Adipocytes were infected at 4, 9, 12, 16, and 20 days post-differentiation (MOI = 1), with measurements taken 48 h post-infection. ( E ) Viral loads measured by plaque assay; data represent two independent experiments ( n = 4, biological replicates, **** p < 0.0001). Statistical analysis was performed using one-way ANOVA. ( F ) Western blot of viral nucleocapsid, FABP4, and β-actin proteins levels in cell lysates. ( G ) Quantification of nucleocapsid and FABP4 band intensities normalized to β-actin, representative of two independent experiments ( n = 4, biological replicates, **** p < 0.0001, *** p = 0.0001 and 0.0006). Statistical analysis was performed using two-way ANOVA. ( H , I ) Percent area of lipid droplets in infected cells and neighboring cells, quantified by fluorescence neutral lipid staining (Bodipy). Infected cells identified by ( H ) nucleocapsid-positive staining and ( I ) dsRNA-positive staining. Data pooled from two independent experiments ( n = 6, biological replicates, **** p < 0.0001, *** p = 0.0001); statistical analysis was performed using a standard t-test. ( J ) Representative confocal images of infected differentiated adipocytes (MOI = 1), stained for dsRNA (red), lipid droplets (yellow), and nucleus (DAPI, blue). Scale bar = 50 μm; magnified regions = 10 μm ( n = 3). ( K ) Percent lipid droplet area relative to dsRNA-positive area per cell. Pearson correlation coefficient indicated as r. ( L , M ) Representative confocal images of control and infected adipocytes at 8, 12, 24, and 48 h post-infection, stained for dsRNA (red), lipid droplets (yellow), calnexin (blue), and FABP4 (gray). ( L ) Merged image of all stains (Scale bar = 50 μm); insets highlight overlap of FABP4, dsRNA, and calnexin (Scale bar = 5 μm). ( M ) Signal overlap between dsRNA, FABP4, and calnexin, and between FABP4 and calnexin individually (Scale bar = 5 μm). ( N – P ) Colocalization of target signals over time, represented as Pearson correlation R. Cells infected with MOI = 3 at 8 and 12 hpi; data pooled from two independent experiments ( n = 6, biological replicates, **** p < 0.0001). For 24 and 48 hpi, MOI = 1 was used ( n = 3) biological replicates. Statistical analysis was performed using one-way ANOVA. Data shown as mean ± s.e.m. .
Htert Human Pre Adipocytes, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+preadipocytes/hTERT+A41hWAT-SVF%3B+Preadipocyte%3B+Human/pmc11904229-32-0-4
Average 92 stars, based on 1 article reviews
htert human pre adipocytes - by Bioz Stars, 2026-09
92/100 stars
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pre adipocyte growth medium  (Cell Applications Inc)
94
Cell Applications Inc pre adipocyte growth medium
( A – C ) <t>hTERT</t> pre-adipocytes and differentiated adipocytes infected with SARS-CoV-2 (WA1/2020, MOI = 1). ( A , B ) Relative expression of viral ( A ) genomic RNA (nucleocapsid, *** p = 0.0002, ** p = 0.0018) and ( B ) sub-genomic RNA (ORF1ab, *** p = 0.0003, * p = 0.0263), normalized to β-actin. ( C ) Viral loads measured from supernatant using plaque assay. (**** p < 0.0001, ** p = 0.0024). Data are pooled from two independent experiments ( n = 8, biological replicates). Statistical analysis was performed using two-way ANOVA. ( D ) IL-6 levels in the supernatant of differentiated adipocytes with or without viral infection (MOI = 1), measured by ELISA. Data are pooled from three independent experiments ( n = 14, biological replicates, **** p < 0.0001). Statistical analysis was performed using one-way ANOVA. ( E – G ) Adipocytes were infected at 4, 9, 12, 16, and 20 days post-differentiation (MOI = 1), with measurements taken 48 h post-infection. ( E ) Viral loads measured by plaque assay; data represent two independent experiments ( n = 4, biological replicates, **** p < 0.0001). Statistical analysis was performed using one-way ANOVA. ( F ) Western blot of viral nucleocapsid, FABP4, and β-actin proteins levels in cell lysates. ( G ) Quantification of nucleocapsid and FABP4 band intensities normalized to β-actin, representative of two independent experiments ( n = 4, biological replicates, **** p < 0.0001, *** p = 0.0001 and 0.0006). Statistical analysis was performed using two-way ANOVA. ( H , I ) Percent area of lipid droplets in infected cells and neighboring cells, quantified by fluorescence neutral lipid staining (Bodipy). Infected cells identified by ( H ) nucleocapsid-positive staining and ( I ) dsRNA-positive staining. Data pooled from two independent experiments ( n = 6, biological replicates, **** p < 0.0001, *** p = 0.0001); statistical analysis was performed using a standard t-test. ( J ) Representative confocal images of infected differentiated adipocytes (MOI = 1), stained for dsRNA (red), lipid droplets (yellow), and nucleus (DAPI, blue). Scale bar = 50 μm; magnified regions = 10 μm ( n = 3). ( K ) Percent lipid droplet area relative to dsRNA-positive area per cell. Pearson correlation coefficient indicated as r. ( L , M ) Representative confocal images of control and infected adipocytes at 8, 12, 24, and 48 h post-infection, stained for dsRNA (red), lipid droplets (yellow), calnexin (blue), and FABP4 (gray). ( L ) Merged image of all stains (Scale bar = 50 μm); insets highlight overlap of FABP4, dsRNA, and calnexin (Scale bar = 5 μm). ( M ) Signal overlap between dsRNA, FABP4, and calnexin, and between FABP4 and calnexin individually (Scale bar = 5 μm). ( N – P ) Colocalization of target signals over time, represented as Pearson correlation R. Cells infected with MOI = 3 at 8 and 12 hpi; data pooled from two independent experiments ( n = 6, biological replicates, **** p < 0.0001). For 24 and 48 hpi, MOI = 1 was used ( n = 3) biological replicates. Statistical analysis was performed using one-way ANOVA. Data shown as mean ± s.e.m. .
Pre Adipocyte Growth Medium, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+preadipocytes/Human+Preadipocyte+Media/pm42025693-72-7-10
Average 94 stars, based on 1 article reviews
pre adipocyte growth medium - by Bioz Stars, 2026-09
94/100 stars
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adipocytes differentiation media 130  (Cell Applications Inc)
94
Cell Applications Inc adipocytes differentiation media 130
( A – C ) <t>hTERT</t> pre-adipocytes and differentiated adipocytes infected with SARS-CoV-2 (WA1/2020, MOI = 1). ( A , B ) Relative expression of viral ( A ) genomic RNA (nucleocapsid, *** p = 0.0002, ** p = 0.0018) and ( B ) sub-genomic RNA (ORF1ab, *** p = 0.0003, * p = 0.0263), normalized to β-actin. ( C ) Viral loads measured from supernatant using plaque assay. (**** p < 0.0001, ** p = 0.0024). Data are pooled from two independent experiments ( n = 8, biological replicates). Statistical analysis was performed using two-way ANOVA. ( D ) IL-6 levels in the supernatant of differentiated adipocytes with or without viral infection (MOI = 1), measured by ELISA. Data are pooled from three independent experiments ( n = 14, biological replicates, **** p < 0.0001). Statistical analysis was performed using one-way ANOVA. ( E – G ) Adipocytes were infected at 4, 9, 12, 16, and 20 days post-differentiation (MOI = 1), with measurements taken 48 h post-infection. ( E ) Viral loads measured by plaque assay; data represent two independent experiments ( n = 4, biological replicates, **** p < 0.0001). Statistical analysis was performed using one-way ANOVA. ( F ) Western blot of viral nucleocapsid, FABP4, and β-actin proteins levels in cell lysates. ( G ) Quantification of nucleocapsid and FABP4 band intensities normalized to β-actin, representative of two independent experiments ( n = 4, biological replicates, **** p < 0.0001, *** p = 0.0001 and 0.0006). Statistical analysis was performed using two-way ANOVA. ( H , I ) Percent area of lipid droplets in infected cells and neighboring cells, quantified by fluorescence neutral lipid staining (Bodipy). Infected cells identified by ( H ) nucleocapsid-positive staining and ( I ) dsRNA-positive staining. Data pooled from two independent experiments ( n = 6, biological replicates, **** p < 0.0001, *** p = 0.0001); statistical analysis was performed using a standard t-test. ( J ) Representative confocal images of infected differentiated adipocytes (MOI = 1), stained for dsRNA (red), lipid droplets (yellow), and nucleus (DAPI, blue). Scale bar = 50 μm; magnified regions = 10 μm ( n = 3). ( K ) Percent lipid droplet area relative to dsRNA-positive area per cell. Pearson correlation coefficient indicated as r. ( L , M ) Representative confocal images of control and infected adipocytes at 8, 12, 24, and 48 h post-infection, stained for dsRNA (red), lipid droplets (yellow), calnexin (blue), and FABP4 (gray). ( L ) Merged image of all stains (Scale bar = 50 μm); insets highlight overlap of FABP4, dsRNA, and calnexin (Scale bar = 5 μm). ( M ) Signal overlap between dsRNA, FABP4, and calnexin, and between FABP4 and calnexin individually (Scale bar = 5 μm). ( N – P ) Colocalization of target signals over time, represented as Pearson correlation R. Cells infected with MOI = 3 at 8 and 12 hpi; data pooled from two independent experiments ( n = 6, biological replicates, **** p < 0.0001). For 24 and 48 hpi, MOI = 1 was used ( n = 3) biological replicates. Statistical analysis was performed using one-way ANOVA. Data shown as mean ± s.e.m. .
Adipocytes Differentiation Media 130, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+preadipocytes/Human+Preadipocyte+Differentiation+Medium/pm42025693-73-7-11
Average 94 stars, based on 1 article reviews
adipocytes differentiation media 130 - by Bioz Stars, 2026-09
94/100 stars
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preadipocyte growth medium  (Cell Applications Inc)
92
Cell Applications Inc preadipocyte growth medium
( A – C ) <t>hTERT</t> pre-adipocytes and differentiated adipocytes infected with SARS-CoV-2 (WA1/2020, MOI = 1). ( A , B ) Relative expression of viral ( A ) genomic RNA (nucleocapsid, *** p = 0.0002, ** p = 0.0018) and ( B ) sub-genomic RNA (ORF1ab, *** p = 0.0003, * p = 0.0263), normalized to β-actin. ( C ) Viral loads measured from supernatant using plaque assay. (**** p < 0.0001, ** p = 0.0024). Data are pooled from two independent experiments ( n = 8, biological replicates). Statistical analysis was performed using two-way ANOVA. ( D ) IL-6 levels in the supernatant of differentiated adipocytes with or without viral infection (MOI = 1), measured by ELISA. Data are pooled from three independent experiments ( n = 14, biological replicates, **** p < 0.0001). Statistical analysis was performed using one-way ANOVA. ( E – G ) Adipocytes were infected at 4, 9, 12, 16, and 20 days post-differentiation (MOI = 1), with measurements taken 48 h post-infection. ( E ) Viral loads measured by plaque assay; data represent two independent experiments ( n = 4, biological replicates, **** p < 0.0001). Statistical analysis was performed using one-way ANOVA. ( F ) Western blot of viral nucleocapsid, FABP4, and β-actin proteins levels in cell lysates. ( G ) Quantification of nucleocapsid and FABP4 band intensities normalized to β-actin, representative of two independent experiments ( n = 4, biological replicates, **** p < 0.0001, *** p = 0.0001 and 0.0006). Statistical analysis was performed using two-way ANOVA. ( H , I ) Percent area of lipid droplets in infected cells and neighboring cells, quantified by fluorescence neutral lipid staining (Bodipy). Infected cells identified by ( H ) nucleocapsid-positive staining and ( I ) dsRNA-positive staining. Data pooled from two independent experiments ( n = 6, biological replicates, **** p < 0.0001, *** p = 0.0001); statistical analysis was performed using a standard t-test. ( J ) Representative confocal images of infected differentiated adipocytes (MOI = 1), stained for dsRNA (red), lipid droplets (yellow), and nucleus (DAPI, blue). Scale bar = 50 μm; magnified regions = 10 μm ( n = 3). ( K ) Percent lipid droplet area relative to dsRNA-positive area per cell. Pearson correlation coefficient indicated as r. ( L , M ) Representative confocal images of control and infected adipocytes at 8, 12, 24, and 48 h post-infection, stained for dsRNA (red), lipid droplets (yellow), calnexin (blue), and FABP4 (gray). ( L ) Merged image of all stains (Scale bar = 50 μm); insets highlight overlap of FABP4, dsRNA, and calnexin (Scale bar = 5 μm). ( M ) Signal overlap between dsRNA, FABP4, and calnexin, and between FABP4 and calnexin individually (Scale bar = 5 μm). ( N – P ) Colocalization of target signals over time, represented as Pearson correlation R. Cells infected with MOI = 3 at 8 and 12 hpi; data pooled from two independent experiments ( n = 6, biological replicates, **** p < 0.0001). For 24 and 48 hpi, MOI = 1 was used ( n = 3) biological replicates. Statistical analysis was performed using one-way ANOVA. Data shown as mean ± s.e.m. .
Preadipocyte Growth Medium, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+preadipocytes/Human+Brown+Fat+Preadipocyte+Medium/10__1074_slash_jbc__m115__698902-231-10-16
Average 92 stars, based on 1 article reviews
preadipocyte growth medium - by Bioz Stars, 2026-09
92/100 stars
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heart tissue catalog no 802h 05a cell applications san diego ca  (Cell Applications Inc)
94
Cell Applications Inc heart tissue catalog no 802h 05a cell applications san diego ca
( A – C ) <t>hTERT</t> pre-adipocytes and differentiated adipocytes infected with SARS-CoV-2 (WA1/2020, MOI = 1). ( A , B ) Relative expression of viral ( A ) genomic RNA (nucleocapsid, *** p = 0.0002, ** p = 0.0018) and ( B ) sub-genomic RNA (ORF1ab, *** p = 0.0003, * p = 0.0263), normalized to β-actin. ( C ) Viral loads measured from supernatant using plaque assay. (**** p < 0.0001, ** p = 0.0024). Data are pooled from two independent experiments ( n = 8, biological replicates). Statistical analysis was performed using two-way ANOVA. ( D ) IL-6 levels in the supernatant of differentiated adipocytes with or without viral infection (MOI = 1), measured by ELISA. Data are pooled from three independent experiments ( n = 14, biological replicates, **** p < 0.0001). Statistical analysis was performed using one-way ANOVA. ( E – G ) Adipocytes were infected at 4, 9, 12, 16, and 20 days post-differentiation (MOI = 1), with measurements taken 48 h post-infection. ( E ) Viral loads measured by plaque assay; data represent two independent experiments ( n = 4, biological replicates, **** p < 0.0001). Statistical analysis was performed using one-way ANOVA. ( F ) Western blot of viral nucleocapsid, FABP4, and β-actin proteins levels in cell lysates. ( G ) Quantification of nucleocapsid and FABP4 band intensities normalized to β-actin, representative of two independent experiments ( n = 4, biological replicates, **** p < 0.0001, *** p = 0.0001 and 0.0006). Statistical analysis was performed using two-way ANOVA. ( H , I ) Percent area of lipid droplets in infected cells and neighboring cells, quantified by fluorescence neutral lipid staining (Bodipy). Infected cells identified by ( H ) nucleocapsid-positive staining and ( I ) dsRNA-positive staining. Data pooled from two independent experiments ( n = 6, biological replicates, **** p < 0.0001, *** p = 0.0001); statistical analysis was performed using a standard t-test. ( J ) Representative confocal images of infected differentiated adipocytes (MOI = 1), stained for dsRNA (red), lipid droplets (yellow), and nucleus (DAPI, blue). Scale bar = 50 μm; magnified regions = 10 μm ( n = 3). ( K ) Percent lipid droplet area relative to dsRNA-positive area per cell. Pearson correlation coefficient indicated as r. ( L , M ) Representative confocal images of control and infected adipocytes at 8, 12, 24, and 48 h post-infection, stained for dsRNA (red), lipid droplets (yellow), calnexin (blue), and FABP4 (gray). ( L ) Merged image of all stains (Scale bar = 50 μm); insets highlight overlap of FABP4, dsRNA, and calnexin (Scale bar = 5 μm). ( M ) Signal overlap between dsRNA, FABP4, and calnexin, and between FABP4 and calnexin individually (Scale bar = 5 μm). ( N – P ) Colocalization of target signals over time, represented as Pearson correlation R. Cells infected with MOI = 3 at 8 and 12 hpi; data pooled from two independent experiments ( n = 6, biological replicates, **** p < 0.0001). For 24 and 48 hpi, MOI = 1 was used ( n = 3) biological replicates. Statistical analysis was performed using one-way ANOVA. Data shown as mean ± s.e.m. .
Heart Tissue Catalog No 802h 05a Cell Applications San Diego Ca, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+preadipocytes/Human+Preadipocytes%3A+HPAd/ppr0535575-37-22-27
Average 94 stars, based on 1 article reviews
heart tissue catalog no 802h 05a cell applications san diego ca - by Bioz Stars, 2026-09
94/100 stars
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primary human visceral preadipocytes  (Innoprot Inc)
93
Innoprot Inc primary human visceral preadipocytes
( A – C ) <t>hTERT</t> pre-adipocytes and differentiated adipocytes infected with SARS-CoV-2 (WA1/2020, MOI = 1). ( A , B ) Relative expression of viral ( A ) genomic RNA (nucleocapsid, *** p = 0.0002, ** p = 0.0018) and ( B ) sub-genomic RNA (ORF1ab, *** p = 0.0003, * p = 0.0263), normalized to β-actin. ( C ) Viral loads measured from supernatant using plaque assay. (**** p < 0.0001, ** p = 0.0024). Data are pooled from two independent experiments ( n = 8, biological replicates). Statistical analysis was performed using two-way ANOVA. ( D ) IL-6 levels in the supernatant of differentiated adipocytes with or without viral infection (MOI = 1), measured by ELISA. Data are pooled from three independent experiments ( n = 14, biological replicates, **** p < 0.0001). Statistical analysis was performed using one-way ANOVA. ( E – G ) Adipocytes were infected at 4, 9, 12, 16, and 20 days post-differentiation (MOI = 1), with measurements taken 48 h post-infection. ( E ) Viral loads measured by plaque assay; data represent two independent experiments ( n = 4, biological replicates, **** p < 0.0001). Statistical analysis was performed using one-way ANOVA. ( F ) Western blot of viral nucleocapsid, FABP4, and β-actin proteins levels in cell lysates. ( G ) Quantification of nucleocapsid and FABP4 band intensities normalized to β-actin, representative of two independent experiments ( n = 4, biological replicates, **** p < 0.0001, *** p = 0.0001 and 0.0006). Statistical analysis was performed using two-way ANOVA. ( H , I ) Percent area of lipid droplets in infected cells and neighboring cells, quantified by fluorescence neutral lipid staining (Bodipy). Infected cells identified by ( H ) nucleocapsid-positive staining and ( I ) dsRNA-positive staining. Data pooled from two independent experiments ( n = 6, biological replicates, **** p < 0.0001, *** p = 0.0001); statistical analysis was performed using a standard t-test. ( J ) Representative confocal images of infected differentiated adipocytes (MOI = 1), stained for dsRNA (red), lipid droplets (yellow), and nucleus (DAPI, blue). Scale bar = 50 μm; magnified regions = 10 μm ( n = 3). ( K ) Percent lipid droplet area relative to dsRNA-positive area per cell. Pearson correlation coefficient indicated as r. ( L , M ) Representative confocal images of control and infected adipocytes at 8, 12, 24, and 48 h post-infection, stained for dsRNA (red), lipid droplets (yellow), calnexin (blue), and FABP4 (gray). ( L ) Merged image of all stains (Scale bar = 50 μm); insets highlight overlap of FABP4, dsRNA, and calnexin (Scale bar = 5 μm). ( M ) Signal overlap between dsRNA, FABP4, and calnexin, and between FABP4 and calnexin individually (Scale bar = 5 μm). ( N – P ) Colocalization of target signals over time, represented as Pearson correlation R. Cells infected with MOI = 3 at 8 and 12 hpi; data pooled from two independent experiments ( n = 6, biological replicates, **** p < 0.0001). For 24 and 48 hpi, MOI = 1 was used ( n = 3) biological replicates. Statistical analysis was performed using one-way ANOVA. Data shown as mean ± s.e.m. .
Primary Human Visceral Preadipocytes, supplied by Innoprot Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+preadipocytes/Human+Preadipocytes/10__32383_slash_appdr_slash_192453-40-0-9
Average 93 stars, based on 1 article reviews
primary human visceral preadipocytes - by Bioz Stars, 2026-09
93/100 stars
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human subcutaneous primary adipocytes  (Cell Applications Inc)
94
Cell Applications Inc human subcutaneous primary adipocytes
( A – C ) <t>hTERT</t> pre-adipocytes and differentiated adipocytes infected with SARS-CoV-2 (WA1/2020, MOI = 1). ( A , B ) Relative expression of viral ( A ) genomic RNA (nucleocapsid, *** p = 0.0002, ** p = 0.0018) and ( B ) sub-genomic RNA (ORF1ab, *** p = 0.0003, * p = 0.0263), normalized to β-actin. ( C ) Viral loads measured from supernatant using plaque assay. (**** p < 0.0001, ** p = 0.0024). Data are pooled from two independent experiments ( n = 8, biological replicates). Statistical analysis was performed using two-way ANOVA. ( D ) IL-6 levels in the supernatant of differentiated adipocytes with or without viral infection (MOI = 1), measured by ELISA. Data are pooled from three independent experiments ( n = 14, biological replicates, **** p < 0.0001). Statistical analysis was performed using one-way ANOVA. ( E – G ) Adipocytes were infected at 4, 9, 12, 16, and 20 days post-differentiation (MOI = 1), with measurements taken 48 h post-infection. ( E ) Viral loads measured by plaque assay; data represent two independent experiments ( n = 4, biological replicates, **** p < 0.0001). Statistical analysis was performed using one-way ANOVA. ( F ) Western blot of viral nucleocapsid, FABP4, and β-actin proteins levels in cell lysates. ( G ) Quantification of nucleocapsid and FABP4 band intensities normalized to β-actin, representative of two independent experiments ( n = 4, biological replicates, **** p < 0.0001, *** p = 0.0001 and 0.0006). Statistical analysis was performed using two-way ANOVA. ( H , I ) Percent area of lipid droplets in infected cells and neighboring cells, quantified by fluorescence neutral lipid staining (Bodipy). Infected cells identified by ( H ) nucleocapsid-positive staining and ( I ) dsRNA-positive staining. Data pooled from two independent experiments ( n = 6, biological replicates, **** p < 0.0001, *** p = 0.0001); statistical analysis was performed using a standard t-test. ( J ) Representative confocal images of infected differentiated adipocytes (MOI = 1), stained for dsRNA (red), lipid droplets (yellow), and nucleus (DAPI, blue). Scale bar = 50 μm; magnified regions = 10 μm ( n = 3). ( K ) Percent lipid droplet area relative to dsRNA-positive area per cell. Pearson correlation coefficient indicated as r. ( L , M ) Representative confocal images of control and infected adipocytes at 8, 12, 24, and 48 h post-infection, stained for dsRNA (red), lipid droplets (yellow), calnexin (blue), and FABP4 (gray). ( L ) Merged image of all stains (Scale bar = 50 μm); insets highlight overlap of FABP4, dsRNA, and calnexin (Scale bar = 5 μm). ( M ) Signal overlap between dsRNA, FABP4, and calnexin, and between FABP4 and calnexin individually (Scale bar = 5 μm). ( N – P ) Colocalization of target signals over time, represented as Pearson correlation R. Cells infected with MOI = 3 at 8 and 12 hpi; data pooled from two independent experiments ( n = 6, biological replicates, **** p < 0.0001). For 24 and 48 hpi, MOI = 1 was used ( n = 3) biological replicates. Statistical analysis was performed using one-way ANOVA. Data shown as mean ± s.e.m. .
Human Subcutaneous Primary Adipocytes, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+preadipocytes/Human+Preadipocytes%3A+S-HPAd%3A+Pre-Screened/pm37231626-72-0-17
Average 94 stars, based on 1 article reviews
human subcutaneous primary adipocytes - by Bioz Stars, 2026-09
94/100 stars
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human preadipocytes la  (LaCell L L C)
90
LaCell L L C human preadipocytes la
( A – C ) <t>hTERT</t> pre-adipocytes and differentiated adipocytes infected with SARS-CoV-2 (WA1/2020, MOI = 1). ( A , B ) Relative expression of viral ( A ) genomic RNA (nucleocapsid, *** p = 0.0002, ** p = 0.0018) and ( B ) sub-genomic RNA (ORF1ab, *** p = 0.0003, * p = 0.0263), normalized to β-actin. ( C ) Viral loads measured from supernatant using plaque assay. (**** p < 0.0001, ** p = 0.0024). Data are pooled from two independent experiments ( n = 8, biological replicates). Statistical analysis was performed using two-way ANOVA. ( D ) IL-6 levels in the supernatant of differentiated adipocytes with or without viral infection (MOI = 1), measured by ELISA. Data are pooled from three independent experiments ( n = 14, biological replicates, **** p < 0.0001). Statistical analysis was performed using one-way ANOVA. ( E – G ) Adipocytes were infected at 4, 9, 12, 16, and 20 days post-differentiation (MOI = 1), with measurements taken 48 h post-infection. ( E ) Viral loads measured by plaque assay; data represent two independent experiments ( n = 4, biological replicates, **** p < 0.0001). Statistical analysis was performed using one-way ANOVA. ( F ) Western blot of viral nucleocapsid, FABP4, and β-actin proteins levels in cell lysates. ( G ) Quantification of nucleocapsid and FABP4 band intensities normalized to β-actin, representative of two independent experiments ( n = 4, biological replicates, **** p < 0.0001, *** p = 0.0001 and 0.0006). Statistical analysis was performed using two-way ANOVA. ( H , I ) Percent area of lipid droplets in infected cells and neighboring cells, quantified by fluorescence neutral lipid staining (Bodipy). Infected cells identified by ( H ) nucleocapsid-positive staining and ( I ) dsRNA-positive staining. Data pooled from two independent experiments ( n = 6, biological replicates, **** p < 0.0001, *** p = 0.0001); statistical analysis was performed using a standard t-test. ( J ) Representative confocal images of infected differentiated adipocytes (MOI = 1), stained for dsRNA (red), lipid droplets (yellow), and nucleus (DAPI, blue). Scale bar = 50 μm; magnified regions = 10 μm ( n = 3). ( K ) Percent lipid droplet area relative to dsRNA-positive area per cell. Pearson correlation coefficient indicated as r. ( L , M ) Representative confocal images of control and infected adipocytes at 8, 12, 24, and 48 h post-infection, stained for dsRNA (red), lipid droplets (yellow), calnexin (blue), and FABP4 (gray). ( L ) Merged image of all stains (Scale bar = 50 μm); insets highlight overlap of FABP4, dsRNA, and calnexin (Scale bar = 5 μm). ( M ) Signal overlap between dsRNA, FABP4, and calnexin, and between FABP4 and calnexin individually (Scale bar = 5 μm). ( N – P ) Colocalization of target signals over time, represented as Pearson correlation R. Cells infected with MOI = 3 at 8 and 12 hpi; data pooled from two independent experiments ( n = 6, biological replicates, **** p < 0.0001). For 24 and 48 hpi, MOI = 1 was used ( n = 3) biological replicates. Statistical analysis was performed using one-way ANOVA. Data shown as mean ± s.e.m. .
Human Preadipocytes La, supplied by LaCell L L C, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+preadipocytes/human+preadipocytes+la/10__1089_slash_biores__2015__0028-74-3-7
Average 90 stars, based on 1 article reviews
human preadipocytes la - by Bioz Stars, 2026-09
90/100 stars
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human visceral preadipocytes  (ScienCell)
90
ScienCell human visceral preadipocytes
( A – C ) <t>hTERT</t> pre-adipocytes and differentiated adipocytes infected with SARS-CoV-2 (WA1/2020, MOI = 1). ( A , B ) Relative expression of viral ( A ) genomic RNA (nucleocapsid, *** p = 0.0002, ** p = 0.0018) and ( B ) sub-genomic RNA (ORF1ab, *** p = 0.0003, * p = 0.0263), normalized to β-actin. ( C ) Viral loads measured from supernatant using plaque assay. (**** p < 0.0001, ** p = 0.0024). Data are pooled from two independent experiments ( n = 8, biological replicates). Statistical analysis was performed using two-way ANOVA. ( D ) IL-6 levels in the supernatant of differentiated adipocytes with or without viral infection (MOI = 1), measured by ELISA. Data are pooled from three independent experiments ( n = 14, biological replicates, **** p < 0.0001). Statistical analysis was performed using one-way ANOVA. ( E – G ) Adipocytes were infected at 4, 9, 12, 16, and 20 days post-differentiation (MOI = 1), with measurements taken 48 h post-infection. ( E ) Viral loads measured by plaque assay; data represent two independent experiments ( n = 4, biological replicates, **** p < 0.0001). Statistical analysis was performed using one-way ANOVA. ( F ) Western blot of viral nucleocapsid, FABP4, and β-actin proteins levels in cell lysates. ( G ) Quantification of nucleocapsid and FABP4 band intensities normalized to β-actin, representative of two independent experiments ( n = 4, biological replicates, **** p < 0.0001, *** p = 0.0001 and 0.0006). Statistical analysis was performed using two-way ANOVA. ( H , I ) Percent area of lipid droplets in infected cells and neighboring cells, quantified by fluorescence neutral lipid staining (Bodipy). Infected cells identified by ( H ) nucleocapsid-positive staining and ( I ) dsRNA-positive staining. Data pooled from two independent experiments ( n = 6, biological replicates, **** p < 0.0001, *** p = 0.0001); statistical analysis was performed using a standard t-test. ( J ) Representative confocal images of infected differentiated adipocytes (MOI = 1), stained for dsRNA (red), lipid droplets (yellow), and nucleus (DAPI, blue). Scale bar = 50 μm; magnified regions = 10 μm ( n = 3). ( K ) Percent lipid droplet area relative to dsRNA-positive area per cell. Pearson correlation coefficient indicated as r. ( L , M ) Representative confocal images of control and infected adipocytes at 8, 12, 24, and 48 h post-infection, stained for dsRNA (red), lipid droplets (yellow), calnexin (blue), and FABP4 (gray). ( L ) Merged image of all stains (Scale bar = 50 μm); insets highlight overlap of FABP4, dsRNA, and calnexin (Scale bar = 5 μm). ( M ) Signal overlap between dsRNA, FABP4, and calnexin, and between FABP4 and calnexin individually (Scale bar = 5 μm). ( N – P ) Colocalization of target signals over time, represented as Pearson correlation R. Cells infected with MOI = 3 at 8 and 12 hpi; data pooled from two independent experiments ( n = 6, biological replicates, **** p < 0.0001). For 24 and 48 hpi, MOI = 1 was used ( n = 3) biological replicates. Statistical analysis was performed using one-way ANOVA. Data shown as mean ± s.e.m. .
Human Visceral Preadipocytes, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human primary adipocytes (hpa)  (ScienCell)
90
ScienCell human primary adipocytes (hpa)
( A – C ) <t>hTERT</t> pre-adipocytes and differentiated adipocytes infected with SARS-CoV-2 (WA1/2020, MOI = 1). ( A , B ) Relative expression of viral ( A ) genomic RNA (nucleocapsid, *** p = 0.0002, ** p = 0.0018) and ( B ) sub-genomic RNA (ORF1ab, *** p = 0.0003, * p = 0.0263), normalized to β-actin. ( C ) Viral loads measured from supernatant using plaque assay. (**** p < 0.0001, ** p = 0.0024). Data are pooled from two independent experiments ( n = 8, biological replicates). Statistical analysis was performed using two-way ANOVA. ( D ) IL-6 levels in the supernatant of differentiated adipocytes with or without viral infection (MOI = 1), measured by ELISA. Data are pooled from three independent experiments ( n = 14, biological replicates, **** p < 0.0001). Statistical analysis was performed using one-way ANOVA. ( E – G ) Adipocytes were infected at 4, 9, 12, 16, and 20 days post-differentiation (MOI = 1), with measurements taken 48 h post-infection. ( E ) Viral loads measured by plaque assay; data represent two independent experiments ( n = 4, biological replicates, **** p < 0.0001). Statistical analysis was performed using one-way ANOVA. ( F ) Western blot of viral nucleocapsid, FABP4, and β-actin proteins levels in cell lysates. ( G ) Quantification of nucleocapsid and FABP4 band intensities normalized to β-actin, representative of two independent experiments ( n = 4, biological replicates, **** p < 0.0001, *** p = 0.0001 and 0.0006). Statistical analysis was performed using two-way ANOVA. ( H , I ) Percent area of lipid droplets in infected cells and neighboring cells, quantified by fluorescence neutral lipid staining (Bodipy). Infected cells identified by ( H ) nucleocapsid-positive staining and ( I ) dsRNA-positive staining. Data pooled from two independent experiments ( n = 6, biological replicates, **** p < 0.0001, *** p = 0.0001); statistical analysis was performed using a standard t-test. ( J ) Representative confocal images of infected differentiated adipocytes (MOI = 1), stained for dsRNA (red), lipid droplets (yellow), and nucleus (DAPI, blue). Scale bar = 50 μm; magnified regions = 10 μm ( n = 3). ( K ) Percent lipid droplet area relative to dsRNA-positive area per cell. Pearson correlation coefficient indicated as r. ( L , M ) Representative confocal images of control and infected adipocytes at 8, 12, 24, and 48 h post-infection, stained for dsRNA (red), lipid droplets (yellow), calnexin (blue), and FABP4 (gray). ( L ) Merged image of all stains (Scale bar = 50 μm); insets highlight overlap of FABP4, dsRNA, and calnexin (Scale bar = 5 μm). ( M ) Signal overlap between dsRNA, FABP4, and calnexin, and between FABP4 and calnexin individually (Scale bar = 5 μm). ( N – P ) Colocalization of target signals over time, represented as Pearson correlation R. Cells infected with MOI = 3 at 8 and 12 hpi; data pooled from two independent experiments ( n = 6, biological replicates, **** p < 0.0001). For 24 and 48 hpi, MOI = 1 was used ( n = 3) biological replicates. Statistical analysis was performed using one-way ANOVA. Data shown as mean ± s.e.m. .
Human Primary Adipocytes (Hpa), supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+preadipocytes/human+visceral+preadipocytes+hpa+v/pmc09136616-40-9-16
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human preadipocytes  (ZenBio)
90
ZenBio human preadipocytes
( A – C ) <t>hTERT</t> pre-adipocytes and differentiated adipocytes infected with SARS-CoV-2 (WA1/2020, MOI = 1). ( A , B ) Relative expression of viral ( A ) genomic RNA (nucleocapsid, *** p = 0.0002, ** p = 0.0018) and ( B ) sub-genomic RNA (ORF1ab, *** p = 0.0003, * p = 0.0263), normalized to β-actin. ( C ) Viral loads measured from supernatant using plaque assay. (**** p < 0.0001, ** p = 0.0024). Data are pooled from two independent experiments ( n = 8, biological replicates). Statistical analysis was performed using two-way ANOVA. ( D ) IL-6 levels in the supernatant of differentiated adipocytes with or without viral infection (MOI = 1), measured by ELISA. Data are pooled from three independent experiments ( n = 14, biological replicates, **** p < 0.0001). Statistical analysis was performed using one-way ANOVA. ( E – G ) Adipocytes were infected at 4, 9, 12, 16, and 20 days post-differentiation (MOI = 1), with measurements taken 48 h post-infection. ( E ) Viral loads measured by plaque assay; data represent two independent experiments ( n = 4, biological replicates, **** p < 0.0001). Statistical analysis was performed using one-way ANOVA. ( F ) Western blot of viral nucleocapsid, FABP4, and β-actin proteins levels in cell lysates. ( G ) Quantification of nucleocapsid and FABP4 band intensities normalized to β-actin, representative of two independent experiments ( n = 4, biological replicates, **** p < 0.0001, *** p = 0.0001 and 0.0006). Statistical analysis was performed using two-way ANOVA. ( H , I ) Percent area of lipid droplets in infected cells and neighboring cells, quantified by fluorescence neutral lipid staining (Bodipy). Infected cells identified by ( H ) nucleocapsid-positive staining and ( I ) dsRNA-positive staining. Data pooled from two independent experiments ( n = 6, biological replicates, **** p < 0.0001, *** p = 0.0001); statistical analysis was performed using a standard t-test. ( J ) Representative confocal images of infected differentiated adipocytes (MOI = 1), stained for dsRNA (red), lipid droplets (yellow), and nucleus (DAPI, blue). Scale bar = 50 μm; magnified regions = 10 μm ( n = 3). ( K ) Percent lipid droplet area relative to dsRNA-positive area per cell. Pearson correlation coefficient indicated as r. ( L , M ) Representative confocal images of control and infected adipocytes at 8, 12, 24, and 48 h post-infection, stained for dsRNA (red), lipid droplets (yellow), calnexin (blue), and FABP4 (gray). ( L ) Merged image of all stains (Scale bar = 50 μm); insets highlight overlap of FABP4, dsRNA, and calnexin (Scale bar = 5 μm). ( M ) Signal overlap between dsRNA, FABP4, and calnexin, and between FABP4 and calnexin individually (Scale bar = 5 μm). ( N – P ) Colocalization of target signals over time, represented as Pearson correlation R. Cells infected with MOI = 3 at 8 and 12 hpi; data pooled from two independent experiments ( n = 6, biological replicates, **** p < 0.0001). For 24 and 48 hpi, MOI = 1 was used ( n = 3) biological replicates. Statistical analysis was performed using one-way ANOVA. Data shown as mean ± s.e.m. .
Human Preadipocytes, supplied by ZenBio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+preadipocytes/human+preadipocytes/pm16865359-52-0-2
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cryoperserved human subcutaneous preadipocyte cells  (ZenBio)
90
ZenBio cryoperserved human subcutaneous preadipocyte cells
( A – C ) <t>hTERT</t> pre-adipocytes and differentiated adipocytes infected with SARS-CoV-2 (WA1/2020, MOI = 1). ( A , B ) Relative expression of viral ( A ) genomic RNA (nucleocapsid, *** p = 0.0002, ** p = 0.0018) and ( B ) sub-genomic RNA (ORF1ab, *** p = 0.0003, * p = 0.0263), normalized to β-actin. ( C ) Viral loads measured from supernatant using plaque assay. (**** p < 0.0001, ** p = 0.0024). Data are pooled from two independent experiments ( n = 8, biological replicates). Statistical analysis was performed using two-way ANOVA. ( D ) IL-6 levels in the supernatant of differentiated adipocytes with or without viral infection (MOI = 1), measured by ELISA. Data are pooled from three independent experiments ( n = 14, biological replicates, **** p < 0.0001). Statistical analysis was performed using one-way ANOVA. ( E – G ) Adipocytes were infected at 4, 9, 12, 16, and 20 days post-differentiation (MOI = 1), with measurements taken 48 h post-infection. ( E ) Viral loads measured by plaque assay; data represent two independent experiments ( n = 4, biological replicates, **** p < 0.0001). Statistical analysis was performed using one-way ANOVA. ( F ) Western blot of viral nucleocapsid, FABP4, and β-actin proteins levels in cell lysates. ( G ) Quantification of nucleocapsid and FABP4 band intensities normalized to β-actin, representative of two independent experiments ( n = 4, biological replicates, **** p < 0.0001, *** p = 0.0001 and 0.0006). Statistical analysis was performed using two-way ANOVA. ( H , I ) Percent area of lipid droplets in infected cells and neighboring cells, quantified by fluorescence neutral lipid staining (Bodipy). Infected cells identified by ( H ) nucleocapsid-positive staining and ( I ) dsRNA-positive staining. Data pooled from two independent experiments ( n = 6, biological replicates, **** p < 0.0001, *** p = 0.0001); statistical analysis was performed using a standard t-test. ( J ) Representative confocal images of infected differentiated adipocytes (MOI = 1), stained for dsRNA (red), lipid droplets (yellow), and nucleus (DAPI, blue). Scale bar = 50 μm; magnified regions = 10 μm ( n = 3). ( K ) Percent lipid droplet area relative to dsRNA-positive area per cell. Pearson correlation coefficient indicated as r. ( L , M ) Representative confocal images of control and infected adipocytes at 8, 12, 24, and 48 h post-infection, stained for dsRNA (red), lipid droplets (yellow), calnexin (blue), and FABP4 (gray). ( L ) Merged image of all stains (Scale bar = 50 μm); insets highlight overlap of FABP4, dsRNA, and calnexin (Scale bar = 5 μm). ( M ) Signal overlap between dsRNA, FABP4, and calnexin, and between FABP4 and calnexin individually (Scale bar = 5 μm). ( N – P ) Colocalization of target signals over time, represented as Pearson correlation R. Cells infected with MOI = 3 at 8 and 12 hpi; data pooled from two independent experiments ( n = 6, biological replicates, **** p < 0.0001). For 24 and 48 hpi, MOI = 1 was used ( n = 3) biological replicates. Statistical analysis was performed using one-way ANOVA. Data shown as mean ± s.e.m. .
Cryoperserved Human Subcutaneous Preadipocyte Cells, supplied by ZenBio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


( A – C ) hTERT pre-adipocytes and differentiated adipocytes infected with SARS-CoV-2 (WA1/2020, MOI = 1). ( A , B ) Relative expression of viral ( A ) genomic RNA (nucleocapsid, *** p = 0.0002, ** p = 0.0018) and ( B ) sub-genomic RNA (ORF1ab, *** p = 0.0003, * p = 0.0263), normalized to β-actin. ( C ) Viral loads measured from supernatant using plaque assay. (**** p < 0.0001, ** p = 0.0024). Data are pooled from two independent experiments ( n = 8, biological replicates). Statistical analysis was performed using two-way ANOVA. ( D ) IL-6 levels in the supernatant of differentiated adipocytes with or without viral infection (MOI = 1), measured by ELISA. Data are pooled from three independent experiments ( n = 14, biological replicates, **** p < 0.0001). Statistical analysis was performed using one-way ANOVA. ( E – G ) Adipocytes were infected at 4, 9, 12, 16, and 20 days post-differentiation (MOI = 1), with measurements taken 48 h post-infection. ( E ) Viral loads measured by plaque assay; data represent two independent experiments ( n = 4, biological replicates, **** p < 0.0001). Statistical analysis was performed using one-way ANOVA. ( F ) Western blot of viral nucleocapsid, FABP4, and β-actin proteins levels in cell lysates. ( G ) Quantification of nucleocapsid and FABP4 band intensities normalized to β-actin, representative of two independent experiments ( n = 4, biological replicates, **** p < 0.0001, *** p = 0.0001 and 0.0006). Statistical analysis was performed using two-way ANOVA. ( H , I ) Percent area of lipid droplets in infected cells and neighboring cells, quantified by fluorescence neutral lipid staining (Bodipy). Infected cells identified by ( H ) nucleocapsid-positive staining and ( I ) dsRNA-positive staining. Data pooled from two independent experiments ( n = 6, biological replicates, **** p < 0.0001, *** p = 0.0001); statistical analysis was performed using a standard t-test. ( J ) Representative confocal images of infected differentiated adipocytes (MOI = 1), stained for dsRNA (red), lipid droplets (yellow), and nucleus (DAPI, blue). Scale bar = 50 μm; magnified regions = 10 μm ( n = 3). ( K ) Percent lipid droplet area relative to dsRNA-positive area per cell. Pearson correlation coefficient indicated as r. ( L , M ) Representative confocal images of control and infected adipocytes at 8, 12, 24, and 48 h post-infection, stained for dsRNA (red), lipid droplets (yellow), calnexin (blue), and FABP4 (gray). ( L ) Merged image of all stains (Scale bar = 50 μm); insets highlight overlap of FABP4, dsRNA, and calnexin (Scale bar = 5 μm). ( M ) Signal overlap between dsRNA, FABP4, and calnexin, and between FABP4 and calnexin individually (Scale bar = 5 μm). ( N – P ) Colocalization of target signals over time, represented as Pearson correlation R. Cells infected with MOI = 3 at 8 and 12 hpi; data pooled from two independent experiments ( n = 6, biological replicates, **** p < 0.0001). For 24 and 48 hpi, MOI = 1 was used ( n = 3) biological replicates. Statistical analysis was performed using one-way ANOVA. Data shown as mean ± s.e.m. .

Journal: EMBO Molecular Medicine

Article Title: FABP4 as a therapeutic host target controlling SARS-CoV-2 infection

doi: 10.1038/s44321-024-00188-x

Figure Lengend Snippet: ( A – C ) hTERT pre-adipocytes and differentiated adipocytes infected with SARS-CoV-2 (WA1/2020, MOI = 1). ( A , B ) Relative expression of viral ( A ) genomic RNA (nucleocapsid, *** p = 0.0002, ** p = 0.0018) and ( B ) sub-genomic RNA (ORF1ab, *** p = 0.0003, * p = 0.0263), normalized to β-actin. ( C ) Viral loads measured from supernatant using plaque assay. (**** p < 0.0001, ** p = 0.0024). Data are pooled from two independent experiments ( n = 8, biological replicates). Statistical analysis was performed using two-way ANOVA. ( D ) IL-6 levels in the supernatant of differentiated adipocytes with or without viral infection (MOI = 1), measured by ELISA. Data are pooled from three independent experiments ( n = 14, biological replicates, **** p < 0.0001). Statistical analysis was performed using one-way ANOVA. ( E – G ) Adipocytes were infected at 4, 9, 12, 16, and 20 days post-differentiation (MOI = 1), with measurements taken 48 h post-infection. ( E ) Viral loads measured by plaque assay; data represent two independent experiments ( n = 4, biological replicates, **** p < 0.0001). Statistical analysis was performed using one-way ANOVA. ( F ) Western blot of viral nucleocapsid, FABP4, and β-actin proteins levels in cell lysates. ( G ) Quantification of nucleocapsid and FABP4 band intensities normalized to β-actin, representative of two independent experiments ( n = 4, biological replicates, **** p < 0.0001, *** p = 0.0001 and 0.0006). Statistical analysis was performed using two-way ANOVA. ( H , I ) Percent area of lipid droplets in infected cells and neighboring cells, quantified by fluorescence neutral lipid staining (Bodipy). Infected cells identified by ( H ) nucleocapsid-positive staining and ( I ) dsRNA-positive staining. Data pooled from two independent experiments ( n = 6, biological replicates, **** p < 0.0001, *** p = 0.0001); statistical analysis was performed using a standard t-test. ( J ) Representative confocal images of infected differentiated adipocytes (MOI = 1), stained for dsRNA (red), lipid droplets (yellow), and nucleus (DAPI, blue). Scale bar = 50 μm; magnified regions = 10 μm ( n = 3). ( K ) Percent lipid droplet area relative to dsRNA-positive area per cell. Pearson correlation coefficient indicated as r. ( L , M ) Representative confocal images of control and infected adipocytes at 8, 12, 24, and 48 h post-infection, stained for dsRNA (red), lipid droplets (yellow), calnexin (blue), and FABP4 (gray). ( L ) Merged image of all stains (Scale bar = 50 μm); insets highlight overlap of FABP4, dsRNA, and calnexin (Scale bar = 5 μm). ( M ) Signal overlap between dsRNA, FABP4, and calnexin, and between FABP4 and calnexin individually (Scale bar = 5 μm). ( N – P ) Colocalization of target signals over time, represented as Pearson correlation R. Cells infected with MOI = 3 at 8 and 12 hpi; data pooled from two independent experiments ( n = 6, biological replicates, **** p < 0.0001). For 24 and 48 hpi, MOI = 1 was used ( n = 3) biological replicates. Statistical analysis was performed using one-way ANOVA. Data shown as mean ± s.e.m. .

Article Snippet: hTERT human pre-adipocytes. , ATCC , CRL-3386.

Techniques: Infection, Expressing, Plaque Assay, Enzyme-linked Immunosorbent Assay, Western Blot, Fluorescence, Staining, Control

Reagents and tools table

Journal: EMBO Molecular Medicine

Article Title: FABP4 as a therapeutic host target controlling SARS-CoV-2 infection

doi: 10.1038/s44321-024-00188-x

Figure Lengend Snippet: Reagents and tools table

Article Snippet: hTERT human pre-adipocytes. , ATCC , CRL-3386.

Techniques: Knock-Out, Sequencing, Recombinant, shRNA, Staining, Electron Microscopy, Plasmid Preparation, Software, Isolation, cDNA Synthesis, Luciferase, Enzyme-linked Immunosorbent Assay